Composite

Part:BBa_K2066015:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-09-01)


plLac0-1+RiboJ+cI-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 944


Design Notes

We made this part for our Ribozyme Characterization project to see the effect of adding a RiboJ (ribozyme insulator) sequence in the 5' UTR on the input-ouput relationship (transfer function).


Source

The promoter, RBS, RiboJ, and cI-GFP sequences were derived and modified from Lou et. al 2012 "Ribozyme-based insulator parts buffer synthetic circuits from genetic context". The UNS sequences are from Torella et al. 2013.

References